acsA [2018-02-13T10:53:16.000Z]

acetyl-CoA synthetase

locus: BSU29680

pI: 5.55

mw: 64.72 kDa

protein length: 572 aa

gene length: 1716 bp

function: utilization of acetate, fatty acids

product: acetyl-CoA synthetase)

essential: no

ec: 6.2.1.1

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.1|Utilization of organic acids]
• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.1|Utilization of lipids] → [category|SW 2.4.1.3|Utilization of fatty acids]

Gene

Coordinates: 3,038,213 → 3,039,931

The protein

Catalyzed reaction/ biological activity

• ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA (according to Swiss-Prot)

Protein family

• ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|48538F67259B25B64A7BC4263E944C7074E8E1B3|YtcI]

Modification

• acetylated on Lys-549 by [protein|17C3F56CE859BCD7A282C144C46CC92D3506ABF6|AcuA], this results in inactivation [Pubmed|18487328], deacetylated by [protein|A0A6CB19191A251BB5600C18E039978A9A34933C|SrtN] and [protein|1255813797A83D835E0656A2AAAD361B5BB2094B|AcuC] deacetylates (and thereby activates) [protein|6671E7D85E99D349679F0E9D825DC035D10FFD2E|AcsA] [Pubmed|19136592]

Structure

• [PDB|2P2F] (from ''Salmonella enterica'', 37% identity) [Pubmed|17497934]

Expression and Regulation

Operons

• genes: [gene|6671E7D85E99D349679F0E9D825DC035D10FFD2E|acsA]-[gene|D50593AB6035B7DFEC76867B4692B069D91DEC10|ytzK]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7913927], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|7913927], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|12618455,18083814], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]

  regulation

  • repressed by glucose (4.5-fold) ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]) [Pubmed|7913927,12850135]

• genes: [gene|6671E7D85E99D349679F0E9D825DC035D10FFD2E|acsA]-[gene|D50593AB6035B7DFEC76867B4692B069D91DEC10|ytzK]-[gene|EEB0CF0AF0707AC1161F150708F7EDDB749B3C42|tyrS]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [pubmed|7913927], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [pubmed|7913927], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [pubmed|12618455,18083814], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]

  regulation

  • repressed by glucose (4.5-fold) ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]) [Pubmed|7913927,12850135]

Biological materials

Mutant

• GP1212 (''acsA''::''kan''), available in [SW|Stülke] lab
• BKE29680 (Δ[gene|6671E7D85E99D349679F0E9D825DC035D10FFD2E|acsA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCCCAAACATCCCCCTT, downstream forward: _UP4_ACAATGGAGGATTAAAAGGC
• BKK29680 (Δ[gene|6671E7D85E99D349679F0E9D825DC035D10FFD2E|acsA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCCCAAACATCCCCCTT, downstream forward: _UP4_ACAATGGAGGATTAAAAGGC

References

Reviews

• 15316652

Original publications

• 7934817,7913927,18083814,17303131,12618455,19136592,18487328,12850135,16855235,22900538,17497934,29093482